Free interactive chemistry · SIM-SPEC-UV-001
UV–Vis and Beer–Lambert
The Beer–Lambert law: absorbance grows with concentration and path length.
Open the interactive simulation →How to use this simulation
- Choose the absorbing species.
- Move the concentration and path-length sliders.
- Read A and the transmittance, and look at the calibration line.
What to notice
- A = εcl — a straight line through the origin.
- Measure A of an unknown and read its concentration off the line.
Try this
Double the path length. What happens to A and to the transmittance?
Try it for free →